pH-Stat Titration: A Rapid Assay for Enzymatic Degradability of Bio-Based Polymers
نویسندگان
چکیده
منابع مشابه
Degradability of Polymers for Implantable Biomedical Devices
Many key components of implantable medical devices are made from polymeric materials. The functions of these materials include structural support, electrical insulation, protection of other materials from the environment of the body, and biocompatibility, as well as other things such as delivery of a therapeutic drug. In such roles, the stability and integrity of the polymer, over what can be a...
متن کاملAssay of carbonic anhydrase by titration at constant pH.
1. A method is described for measuring accurately the initial velocity of the hydration reaction catalysed by the enzyme carbonic anhydrase (EC 4.2.1.1); the method depends on the titration of H(+) ions at constant pH. 2. Human erythrocyte carbonic anhydrase, isoenzyme C, was used to illustrate the method. Under the experimental conditions employed (0 degrees , pH7.0, in the presence of 45mm-so...
متن کاملA rapid enzymatic assay for methotrexate in serum.
A kinetic enzymological assay for methotrexate in serum is described; the results are available after 1.5 hours. The lower limit of sensivity is 50 nmol/1; the day-to-day variation coefficients compare favourably with other procedures (about 7% in the 200-600 nmol/1 range). The method uses purified dihydrofolic acid reductase from bovine liver. The problem of its instability in the assay is ci...
متن کاملBio-Based Polymers with Potential for Biodegradability
A variety of renewable starting materials, such as sugars and polysaccharides, vegetable oils, lignin, pine resin derivatives, and proteins, have so far been investigated for the preparation of bio-based polymers. Among the various sources of bio-based feedstock, vegetable oils are one of the most widely used starting materials in the polymer industry due to their easy availability, low toxicit...
متن کاملRapid gentamicin assay by enzymatic adenylylation.
A method is described for the assay of gentamicin using the enzyme gentamicin adenylyltransferase derived from an R-factor-carrying strain of Escherichia coli. The reaction involves adenylylation of the gentamicin with ((14)C)-ATP to form a radioactively labelled product. The technique is compared with the plate diffusion and the urease methods. The adenylylation technique gives results compara...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Polymers
سال: 2021
ISSN: 2073-4360
DOI: 10.3390/polym13060860